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Refolding of a fully functional flavivirus methyltransferase revealed that S-adenosyl methionine but not S-adenosyl homocysteine is copurified with flavivirus methyltransferase

Methylation of flavivirus RNA is vital for its stability and translation in the infected host cell. This methylation is mediated by the flavivirus methyltransferase (MTase), which methylates the N7 and 2′-O positions of the viral RNA cap by using S-adenosyl-l-methionine (SAM) as a methyl donor. In t...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Protein Sci
Autori principali: Brecher, Matthew B, Li, Zhong, Zhang, Jing, Chen, Hui, Lin, Qishan, Liu, Binbin, Li, Hongmin
Natura: Artigo
Lingua:Inglês
Pubblicazione: Blackwell Publishing Ltd 2015
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282417/
https://ncbi.nlm.nih.gov/pubmed/25352331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2594
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