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SINGLE-TUBE, HIGHLY PARALLEL MUTATION ENRICHMENT IN CANCER GENE PANELS USING TEMPERATURE-TOLERANT-COLD-PCR

BACKGROUND: Multiplexed detection of low-level mutations presents a technical challenge for many technologies, including cancer gene panels used for targeted-re-sequencing. Analysis of mutations below ~2–5% abundance in tumors with heterogeneity, samples with stromal contamination, or biofluids, is...

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Detalles Bibliográficos
Publicado en:Clin Chem
Autores principales: Castellanos-Rizaldos, E., Richardson, K., Lin, R., Wu, G., Makrigiorgos, G. M.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4281501/
https://ncbi.nlm.nih.gov/pubmed/25297854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1373/clinchem.2014.228361
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