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TEMPERATURE-TOLERANT COLD-PCR ELIMINATES TEMPERATURE STRINGENCY AND ENABLES ROBUST MUTATION ENRICHMENT

BACKGROUND: Low-level mutations in clinical tumor samples often reside below mutation detection limits, thus leading to false negatives that may impact clinical diagnosis and patient management. COLD-PCR is a technology that magnifies unknown mutations during PCR, thus enabling downstream mutation d...

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Detalhes bibliográficos
Main Authors: Castellanos-Rizaldos, E., Liu, Pingfang, Milbury, Coren A., Guha, Minakshi, Brisci, Angela, Cremonesi, Laura, Ferrari, Maurizio, Mamon, Harvey, Makrigiorgos, G. Mike
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3418919/
https://ncbi.nlm.nih.gov/pubmed/22587896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1373/clinchem.2012.183095
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