Carregant...

Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase

Molecular cloning is utilized in nearly every facet of biological and medical research. We have developed a method, termed Hot Fusion, to efficiently clone one or multiple DNA fragments into plasmid vectors without the use of ligase. The method is directional, produces seamless junctions and is not...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:PLoS One
Autors principals: Fu, Changlin, Donovan, William P., Shikapwashya-Hasser, Olga, Ye, Xudong, Cole, Robert H.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4281135/
https://ncbi.nlm.nih.gov/pubmed/25551825
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0115318
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!