Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism

Quantitative PCR (Q-PCR) is a fast and efficient tool to quantify target genes. In eukaryotic cells, quantitative reverse transcription-PCR (Q-RT-PCR) is also used to quantify gene expression, with stably expressed housekeeping genes as standards. In bacteria, such stable expression of housekeeping...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Fey, Axel, Eichler, Stefan, Flavier, Sébastien, Christen, Richard, Höfle, Manfred G., Guzmán, Carlos A.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2004
Sujets:
Methods
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427797/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15184165/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.6.3618-3623.2004
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