Construction of Biologically Functional Bacterial Plasmids In Vitro

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genet...

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Bibliografiske detaljer

Udgivet i:	Proc Natl Acad Sci U S A
Principais autores:	Cohen, Stanley N., Chang, Annie C. Y., Boyer, Herbert W., Helling, Robert B.
Format:	Artigo
Sprog:	Inglês
Udgivet:	National Academy of Sciences 1973
Fag:	Biological Sciences: Biochemistry
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427208/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4594039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.11.3240
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