Construction of Biologically Functional Bacterial Plasmids In Vitro

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genet...

詳細記述

書誌詳細

出版年:	Proc Natl Acad Sci U S A
主要な著者:	Cohen, Stanley N., Chang, Annie C. Y., Boyer, Herbert W., Helling, Robert B.
フォーマット:	Artigo
言語:	Inglês
出版事項:	National Academy of Sciences 1973
主題:	Biological Sciences: Biochemistry
オンライン･アクセス:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427208/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4594039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.11.3240
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