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Construction of Biologically Functional Bacterial Plasmids In Vitro

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genet...

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Detalhes bibliográficos
Main Authors: Cohen, Stanley N., Chang, Annie C. Y., Boyer, Herbert W., Helling, Robert B.
Formato: Artigo
Idioma:Inglês
Publicado em: 1973
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC427208/
https://ncbi.nlm.nih.gov/pubmed/4594039
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