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Construction of Biologically Functional Bacterial Plasmids In Vitro

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genet...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Cohen, Stanley N., Chang, Annie C. Y., Boyer, Herbert W., Helling, Robert B.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1973
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427208/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4594039/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.11.3240
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