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Construction of Biologically Functional Bacterial Plasmids In Vitro
The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genet...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1973
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427208/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4594039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.11.3240 |
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