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Construction of Biologically Functional Bacterial Plasmids In Vitro

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genet...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Cohen, Stanley N., Chang, Annie C. Y., Boyer, Herbert W., Helling, Robert B.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1973
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427208/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4594039/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.11.3240
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