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Effect of Circularity and Superhelicity on Transcription from Bacteriophage λ DNA

We studied RNA synthesis in vitro from closed-circular λ DNA molecules with varying degrees of superhelicity. The four circular templates examined had 0, -50, -110, and -160 superhelical turns under the conditions of the transcription assay. We found that the total amount of RNA synthesis increases...

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Detalhes bibliográficos
Main Authors: Botchan, Peter, Wang, James C., Echols, Harrison
Formato: Artigo
Idioma:Inglês
Publicado em: 1973
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC427173/
https://ncbi.nlm.nih.gov/pubmed/4594034
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