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Effect of Circularity and Superhelicity on Transcription from Bacteriophage λ DNA

We studied RNA synthesis in vitro from closed-circular λ DNA molecules with varying degrees of superhelicity. The four circular templates examined had 0, -50, -110, and -160 superhelical turns under the conditions of the transcription assay. We found that the total amount of RNA synthesis increases...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Botchan, Peter, Wang, James C., Echols, Harrison
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1973
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Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427173/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4594034/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.11.3077
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