A Sensitive Gel-based Method Combining Distinct Cyclophellitol-based Probes for the Identification of Acid/Base Residues in Human Retaining β-Glucosidases

Retaining β-exoglucosidases operate by a mechanism in which the key amino acids driving the glycosidic bond hydrolysis act as catalytic acid/base and nucleophile. Recently we designed two distinct classes of fluorescent cyclophellitol-type activity-based probes (ABPs) that exploit this mechanism to...

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Veröffentlicht in:J Biol Chem
Hauptverfasser: Kallemeijn, Wouter W., Witte, Martin D., Voorn-Brouwer, Tineke M., Walvoort, Marthe T. C., Li, Kah-Yee, Codée, Jeroen D. C., van der Marel, Gijsbert A., Boot, Rolf G., Overkleeft, Herman S., Aerts, Johannes M. F. G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2014
Schlagworte:
Protein Structure and Folding
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4271221/
https://ncbi.nlm.nih.gov/pubmed/25344605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.593376
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