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A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila

The development of clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) technologies promises a quantum leap in genome engineering of model organisms. However, CRISPR-mediated gene targeting reports in Drosophila melanogaster are still restricted to a few gene...

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Publicat a:G3 (Bethesda)
Autors principals: Zhang, Xu, Koolhaas, Wouter H., Schnorrer, Frank
Format: Artigo
Idioma:Inglês
Publicat: Genetics Society of America 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4267936/
https://ncbi.nlm.nih.gov/pubmed/25324299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.013979
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