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A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila

The development of clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) technologies promises a quantum leap in genome engineering of model organisms. However, CRISPR-mediated gene targeting reports in Drosophila melanogaster are still restricted to a few gene...

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Detalles Bibliográficos
Publicado en:G3 (Bethesda)
Main Authors: Zhang, Xu, Koolhaas, Wouter H., Schnorrer, Frank
Formato: Artigo
Idioma:Inglês
Publicado: Genetics Society of America 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4267936/
https://ncbi.nlm.nih.gov/pubmed/25324299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.013979
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