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Single-channel multiplexing without melting curve analysis in real-time PCR
Multiplex real-time PCR with quantification of targets in a single fluorescence channel has been the demand in biotechnology industry. Here, we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this techn...
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| Veröffentlicht in: | Sci Rep |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4262828/ https://ncbi.nlm.nih.gov/pubmed/25501038 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07439 |
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