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PCR Multiplexing Based on a Single Fluorescent Channel Using Dynamic Melting Curve Analysis

[Image: see text] Since its invention in 1986, the polymerase chain reaction (PCR), has become a well-established method for the detection and amplification of deoxyribonucleic acid (DNA) with a specific sequence. Incorporating fluorescent probes, known as TaqMan probes, or DNA intercalating dyes, s...

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Bibliografiske detaljer
Udgivet i:ACS Omega
Main Authors: Zhang, Haoqing, Gaňová, Martina, Yan, ZhiQiang, Chang, Honglong, Neužil, Pavel
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2020
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7689941/
https://ncbi.nlm.nih.gov/pubmed/33251461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.0c04766
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