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Single Fluorescence Channel-based Multiplex Detection of Avian Influenza Virus by Quantitative PCR with Intercalating Dye

Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established method for amplification and detection of segments of double-stranded DNA. Incorporation of fluorogenic probe or DNA intercalating dyes (such as SYBR Green) into the PCR mixture allowed real-time reaction m...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Ahberg, Christian D., Manz, Andreas, Neuzil, Pavel
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5155576/
https://ncbi.nlm.nih.gov/pubmed/26088868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep11479
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