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Arrestin Expression in E. coli and Purification
Purified arrestin proteins are necessary for biochemical, biophysical, and crystallographic studies of these versatile regulators of cell signaling. Here we describe a basic protocol for expression in E. coli and purification of tag-free wild type and mutant arrestins. The method includes ammonium s...
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| Publicado no: | Curr Protoc Pharmacol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4260927/ https://ncbi.nlm.nih.gov/pubmed/25446290 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471141755.ph0211s67 |
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