Nicotinamidase/pyrazinamidase of Mycobacterium tuberculosis forms homo-dimers stabilized by disulfide bonds

Recombinant wild-pyrazinamidase from H37Rv M. tuberculosis was analyzed by gel electrophoresis under differential reducing conditions to evaluate its quaternary structure. PZAse was fractionated by size exclusion chromatography under non-reducing conditions. PZAse activity was measured and mass spec...

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Bibliografiske detaljer
Udgivet i:Tuberculosis (Edinb)
Main Authors: Rueda, Daniel, Sheen, Patricia, Gilman, Robert H., Bueno, Carlos, Santos, Marco, Pando-Robles, Victoria, Batista, Cesar V., Zimic, Mirko
Format: Artigo
Sprog:Inglês
Udgivet: 2014
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4258149/
https://ncbi.nlm.nih.gov/pubmed/25199451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.tube.2014.08.008
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