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A “Proteomic Ruler” for Protein Copy Number and Concentration Estimation without Spike-in Standards
Absolute protein quantification using mass spectrometry (MS)-based proteomics delivers protein concentrations or copy numbers per cell. Existing methodologies typically require a combination of isotope-labeled spike-in references, cell counting, and protein concentration measurements. Here we presen...
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| Udgivet i: | Mol Cell Proteomics |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The American Society for Biochemistry and Molecular Biology
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4256500/ https://ncbi.nlm.nih.gov/pubmed/25225357 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M113.037309 |
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