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Catalytically Relevant Electrostatic Interactions of Cytochrome P450c17 (CYP17A1) and Cytochrome b(5)

Two acidic residues, Glu-48 and Glu-49, of cytochrome b(5) (b(5)) are essential for stimulating the 17,20-lyase activity of cytochrome P450c17 (CYP17A1). Substitution of Ala, Gly, Cys, or Gln for these two glutamic acid residues abrogated all capacity to stimulate 17,20-lyase activity. Mutations E49...

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Dades bibliogràfiques
Publicat a:J Biol Chem
Autors principals: Peng, Hwei-Ming, Liu, Jiayan, Forsberg, Sarah E., Tran, Hong T., Anderson, Sean M., Auchus, Richard J.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4256320/
https://ncbi.nlm.nih.gov/pubmed/25315771
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.608919
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