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Spatially Defined EGF Receptor Activation Reveals an F-Actin-Dependent Phospho-Erk Signaling Complex

We investigated the association of signaling proteins with epidermal growth factor (EGF) receptors (EGFR) using biotinylated EGF bound to streptavidin that is covalently coupled in an ordered array of micron-sized features on silicon surfaces. Using NIH-3T3 cells stably expressing EGFR, we observe c...

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Bibliografske podrobnosti
izdano v:Biophys J
Main Authors: Singhai, Amit, Wakefield, Devin L., Bryant, Kirsten L., Hammes, Stephen R., Holowka, David, Baird, Barbara
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4255200/
https://ncbi.nlm.nih.gov/pubmed/25468343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.09.048
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