Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells

AIM: To find a cost-effective method of preparation of short interfering RNAs based on cloning, fermentation, digestion and purification (CFDP) and test its feasibility to inhibit hepatitis B virus replication in cell culture. METHODS: We constructed an expression vector containing T7 and tac promot...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:World J Gastroenterol
Egile Nagusiak: Qian, Zhi-Kang, Xuan, Bao-Qin, Min, Tai-Shan, Xu, Jian-Feng, Li, Lin, Huang, Wei-Da
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Baishideng Publishing Group Inc 2005
Gaiak:
Viral Hepatitis
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4250676/
https://ncbi.nlm.nih.gov/pubmed/15761967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i9.1297
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