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Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells
AIM: To find a cost-effective method of preparation of short interfering RNAs based on cloning, fermentation, digestion and purification (CFDP) and test its feasibility to inhibit hepatitis B virus replication in cell culture. METHODS: We constructed an expression vector containing T7 and tac promot...
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| Veröffentlicht in: | World J Gastroenterol |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Baishideng Publishing Group Inc
2005
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4250676/ https://ncbi.nlm.nih.gov/pubmed/15761967 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i9.1297 |
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