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Effect of vector-expressed siRNA on HBV replication in hepatoblastoma cells

AIM: To study the effect of siRNA expressed from DNA vector on HBV replication. METHODS: Human U6 promoter was amplified from genomic DNA and cloned into plasmid pUC18 to construct a mammalian siRNA expression vector pUC18U6. Then oligonucleotides coding for a short hairpin RNA against HBV were clon...

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Detalhes bibliográficos
Publicado no:World J Gastroenterol
Main Authors: Liu, Jun, Guo, Ying, Xue, Cai-Fang, Li, Ying-Hui, Huang, Yu-Xiao, Ding, Jin, Gong, Wei-Dong, Zhao, Ya
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Inc 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4572226/
https://ncbi.nlm.nih.gov/pubmed/15222032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i13.1898
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