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Characterization of an Acidic Chitinase from Seeds of Black Soybean (Glycine max (L) Merr Tainan No. 3)

Using 4-methylumbelliferyl-β-D-N,N′,N″-triacetylchitotrioside (4-MU-GlcNAc(3)) as a substrate, an acidic chitinase was purified from seeds of black soybean (Glycine max Tainan no. 3) by ammonium sulfate fractionation and three successive steps of column chromatography. The purified chitinase was a m...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Chang, Ya-Min, Chen, Li-Chun, Wang, Hsin-Yi, Chiang, Chui-Liang, Chang, Chen-Tien, Chung, Yun-Chin
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4249961/
https://ncbi.nlm.nih.gov/pubmed/25437446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0113596
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