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A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing

BACKGROUND: CRISPR-Cas9 is a revolutionary genome editing technique that allows for efficient and directed alterations of the eukaryotic genome. This relatively new technology has already been used in a large number of ‘loss of function’ experiments in cultured cells. Despite its simplicity and effi...

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Bibliografski detalji
Izdano u:BMC Genomics
Glavni autori: Bell, Charles C, Magor, Graham W, Gillinder, Kevin R, Perkins, Andrew C
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2014
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4246457/
https://ncbi.nlm.nih.gov/pubmed/25409780
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-15-1002
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