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SHAPE-Seq 2.0: systematic optimization and extension of high-throughput chemical probing of RNA secondary structure with next generation sequencing

RNA structure is a primary determinant of its function, and methods that merge chemical probing with next generation sequencing have created breakthroughs in the throughput and scale of RNA structure characterization. However, little work has been done to examine the effects of library preparation a...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Loughrey, David, Watters, Kyle E., Settle, Alexander H., Lucks, Julius B.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4245970/
https://ncbi.nlm.nih.gov/pubmed/25303992
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku909
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