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Construction of leaky strains and extracellular production of exogenous proteins in recombinant Escherichia coli

In this study, a strategy of the construction of leaky strains for the extracellular production of target proteins was exploited, in which the genes mrcA, mrcB, pal and lpp (as a control) from Escherichia coli were knocked out by using single- and/or double-gene deletion methods. Then the recombinan...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Microb Biotechnol
Hauptverfasser: Chen, Zhao-Yuan, Cao, Jie, Xie, Li, Li, Xiao-Fei, Yu, Zhen-Hai, Tong, Wang-Yu
Format: Artigo
Sprache:Inglês
Veröffentlicht: BlackWell Publishing Ltd 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4241728/
https://ncbi.nlm.nih.gov/pubmed/24779863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.12127
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