Recombinational construction in Escherichia coli of infectious adenoviral genomes

A two-step gene replacement procedure was developed that generates infectious adenoviral genomes through homologous recombination in Escherichia coli. As a prerequisite, a human adenovirus serotype 5 (Ad5)-derived genome was first introduced as a PacI restriction fragment into an incP-derived replic...

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Detalhes bibliográficos
Main Authors: Crouzet, Joël, Naudin, Laurent, Orsini, Cécile, Vigne, Emmanuelle, Ferrero, Lucy, Le Roux, Aude, Benoit, Patrick, Latta, Martine, Torrent, Christophe, Branellec, Didier, Denèfle, Patrice, Mayaux, Jean-François, Perricaudet, Michel, Yeh, Patrice
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences of the USA 1997
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC19805/
https://ncbi.nlm.nih.gov/pubmed/9037067
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