Enzymatic generation of peptides flanked by basic amino acids to obtain MS/MS spectra with 2× sequence coverage

RATIONALE: Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues. METHODS: He...

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Detalhes bibliográficos
Publicado no:Rapid Commun Mass Spectrom
Main Authors: Ebhardt, H Alexander, Nan, Jie, Chaulk, Steven G, Fahlman, Richard P, Aebersold, Ruedi
Formato: Artigo
Idioma:Inglês
Publicado em: BlackWell Publishing Ltd 2014
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4241039/
https://ncbi.nlm.nih.gov/pubmed/25380496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/rcm.7069
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