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Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli

BACKGROUND: Neurotrophic factors influence survival, differentiation, proliferation and death of neuronal cells within the central nervous system. Human ciliary neurotrophic factor (hCNTF) has neuroprotective properties and is also known to influence energy balance. Consequently, hCNTF has potential...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Itkonen, Jaakko M, Urtti, Arto, Bird, Louise E, Sarkhel, Sanjay
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4237735/
https://ncbi.nlm.nih.gov/pubmed/25394427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-014-0092-x
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