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A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica
BACKGROUND: Recently developed methods for genome editing in bacteria take advantage of the introduction of double-strand breaks by I-SceI in a mutation cassette to select for cells in which homologous recombination has healed the break and introduced a desired mutation. This elegantly designed meth...
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| Udgivet i: | BMC Biotechnol |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4236582/ https://ncbi.nlm.nih.gov/pubmed/25255806 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-84 |
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