High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica

We present a two-step method for scarless–markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The...

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Vydáno v:Curr Microbiol
Hlavní autoři: Geng, Shizhong, Tian, Qin, An, Shuming, Pan, Zhiming, Chen, Xiang, Jiao, Xinan
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer US 2016
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4853454/
https://ncbi.nlm.nih.gov/pubmed/26883127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00284-016-1002-3
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