A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica

BACKGROUND: Recently developed methods for genome editing in bacteria take advantage of the introduction of double-strand breaks by I-SceI in a mutation cassette to select for cells in which homologous recombination has healed the break and introduced a desired mutation. This elegantly designed meth...

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Dades bibliogràfiques
Publicat a:BMC Biotechnol
Autors principals: Kim, Juhan, Webb, Anthony M, Kershner, Jamie P, Blaskowski, Stephen, Copley, Shelley D
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2014
Matèries:
Methodology Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4236582/
https://ncbi.nlm.nih.gov/pubmed/25255806
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-84
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