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Reconstitution of a minimal mtDNA replisome in vitro
We here reconstitute a minimal mammalian mitochondrial DNA (mtDNA) replisome in vitro. The mtDNA polymerase (POLγ) cannot use double-stranded DNA (dsDNA) as template for DNA synthesis. Similarly, the TWINKLE DNA helicase is unable to unwind longer stretches of dsDNA. In combination, POLγ and TWINKLE...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC423294/ https://ncbi.nlm.nih.gov/pubmed/15167897 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7600257 |
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