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Improved Protein Surface Mapping Using Diethylpyrocarbonate with Mass Spectrometric Detection
The reliability and information content of diethylpyrocarbonate (DEPC) as a covalent probe of protein surface structure has been improved when used appropriately with mass spectrometric detection. Using myoglobin, cytochrome c, and β-2-microglobulin as model protein systems, we demonstrate for the f...
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| Pubblicato in: | Anal Chem |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2008
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4232213/ https://ncbi.nlm.nih.gov/pubmed/18338903 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac701999b |
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