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Evaluating Nonpolar Surface Area and LC/MS Response: An Application for Site Occupancy Measurements for Enzyme Intermediates in Polyketide Biosynthesis
RATIONALE: Site occupancy measurements using LC/MS are reported throughout the literature. However, site occupancy quantification suffers from ionization bias between modified and unmodified peptides containing the active site. In this study, we explore the MS signal as a function of nonpolar surfac...
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| Publicado no: | Rapid Commun Mass Spectrom |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4230892/ https://ncbi.nlm.nih.gov/pubmed/25366398 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/rcm.7051 |
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