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FT-ICR-MS characterization of intermediates in the biosynthesis of the α-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF

There are very few fungal polyketide synthases that have been characterized by mass spectrometry. In this paper we describe the in vitro reconstitution and FT-ICR-MS verification of the full activity of an intact 277 kDa fungal polyketide synthase LovF of the lovastatin biosynthetic pathway. We repo...

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Detalhes bibliográficos
Main Authors: Meehan, Michael J., Xie, Xinkai, Zhao, Xiling, Xu, Wei, Tang, Yi, Dorrestein, Pieter C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3085095/
https://ncbi.nlm.nih.gov/pubmed/21069965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi1014776
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