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Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy

This article describes sample preparation techniques for AFM imaging of DNA and protein–DNA complexes. The approach is based on chemical functionalization of the mica surface with aminopropyl silatrane (APS) to yield an APS-mica surface. This surface binds nucleic acids and nucleoprotein complexes i...

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Udgivet i:Methods Mol Biol
Main Authors: Lyubchenko, Yuri L., Gall, Alexander A., Shlyakhtenko, Luda S.
Format: Artigo
Sprog:Inglês
Udgivet: 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4226758/
https://ncbi.nlm.nih.gov/pubmed/24357372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-776-1_17
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