Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherich...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Liu, Daniel S., Nivón, Lucas G., Richter, Florian, Goldman, Peter J., Deerinck, Thomas J., Yao, Jennifer Z., Richardson, Douglas, Phipps, William S., Ye, Anne Z., Ellisman, Mark H., Drennan, Catherine L., Baker, David, Ting, Alice Y.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 2014
Pynciau:
PNAS Plus
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4217414/
https://ncbi.nlm.nih.gov/pubmed/25313043
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1404736111
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