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Evidence for a Catalytically and Kinetically Competent Enzyme–Substrate Cross-Linked Intermediate in Catalysis by Lipoyl Synthase

[Image: see text] Lipoyl synthase (LS) catalyzes the final step in lipoyl cofactor biosynthesis: the insertion of two sulfur atoms at C6 and C8 of an (N(6)-octanoyl)-lysyl residue on a lipoyl carrier protein (LCP). LS is a member of the radical SAM superfamily, enzymes that use a [4Fe–4S] cluster to...

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Detalhes bibliográficos
Main Authors: Lanz, Nicholas D., Pandelia, Maria-Eirini, Kakar, Elizabeth S., Lee, Kyung-Hoon, Krebs, Carsten, Booker, Squire J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4216189/
https://ncbi.nlm.nih.gov/pubmed/24901788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi500432r
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