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Heterologous expression of active human uridine diphosphate glucuronosyltransferase 1A3 in Chinese hamster lung cells

AIM: To obtain the active human recombinant uridine diphosphate glucuronosyltransferase 1A3 (UGT1A3) enzyme from Chinese hamster lung (CHL) cells. METHODS: The full-length UGT1A3 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using total RNA from human liver as templa...

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Detalhes bibliográficos
Main Authors: Chen, Ya-Kun, Li, Xin, Chen, Shu-Qing, Zeng, Su
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Inc 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4205369/
https://ncbi.nlm.nih.gov/pubmed/15609409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i1.118
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