Real-time measurement of in vitro transcription

We have developed a simple method to measure RNA synthesis in real time. In this technique, transcription reactions are performed in the presence of molecular beacons that possess a 2′-O-methylribonucleotide backbone. These probes become fluorescent as they hybridize to nascent RNA during the course...

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Bibliographic Details
Main Authors: Marras, Salvatore A. E., Gold, Benjamin, Kramer, Fred Russell, Smith, Issar, Tyagi, Sanjay
Format: Artigo
Language:Inglês
Published: Oxford University Press 2004
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC419623/
https://ncbi.nlm.nih.gov/pubmed/15155820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh068
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