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RNAPol-ChIP: a novel application of chromatin immunoprecipitation to the analysis of real-time gene transcription
We describe a procedure, RNAPol-ChIP, to measure actual transcriptional rate. It consists of the detection, by chromatin immunoprecipitation (ChIP), of RNA polymerase II within the coding region of genes. To do this, the DNA immunoprecipitated with polymerase antibodies is analysed by PCR, using an...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC443558/ https://ncbi.nlm.nih.gov/pubmed/15247321 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh091 |
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