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Real-time measurement of in vitro transcription
We have developed a simple method to measure RNA synthesis in real time. In this technique, transcription reactions are performed in the presence of molecular beacons that possess a 2′-O-methylribonucleotide backbone. These probes become fluorescent as they hybridize to nascent RNA during the course...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2004
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC419623/ https://ncbi.nlm.nih.gov/pubmed/15155820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh068 |
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