Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

BACKGROUND: The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10–100 μg of RNA. However, due to their small size, most biopsies do not yield this amount. Several different approaches f...

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Detalhes bibliográficos

Publicado no:	BMC Genomics
Principais autores:	Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, Poustka, Annemarie, Sültmann, Holger
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2004
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC419340/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15119961/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2164-5-29
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