Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

BACKGROUND: The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10–100 μg of RNA. However, due to their small size, most biopsies do not yield this amount. Several different approaches f...

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Main Authors: Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, Poustka, Annemarie, Sültmann, Holger
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2004
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC419340/
https://ncbi.nlm.nih.gov/pubmed/15119961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-5-29
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