Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

BACKGROUND: The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10–100 μg of RNA. However, due to their small size, most biopsies do not yield this amount. Several different approaches f...

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Main Authors: Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, Poustka, Annemarie, Sültmann, Holger
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2004
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC419340/
https://ncbi.nlm.nih.gov/pubmed/15119961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-5-29
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