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High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
We present a rapid and inexpensive high-throughput screening protocol to identify transcriptional regulators of alpha-synuclein, a gene associated with Parkinson's disease. 293T cells are transiently transfected with plasmids from an arrayed ORF expression library, together with luciferase repo...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
MyJove Corporation
2014
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4186351/ https://ncbi.nlm.nih.gov/pubmed/24962249 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50282 |
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