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High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

We present a rapid and inexpensive high-throughput screening protocol to identify transcriptional regulators of alpha-synuclein, a gene associated with Parkinson's disease. 293T cells are transiently transfected with plasmids from an arrayed ORF expression library, together with luciferase repo...

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Detalhes bibliográficos
Main Authors: Baker, Jessica M., Boyce, Frederick M.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4186351/
https://ncbi.nlm.nih.gov/pubmed/24962249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50282
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